MUC1-SEC and MUC1-Y overexpression is associated with inflammation in Sjogrens syndromeReportar como inadecuado

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OBJECTIVES: To evaluate the expression and localizationof MUC1-SEC and MUC1-Y isoforms in labial salivaryglands LSG from Sj€ogren’s syndrome patientsSS patients, as well as their in vitro expression inducedby cytokines.SUBJECTS AND METHODS: Labial salivary gland from27 primary SS patients and 22 non-SS sicca subjectswere studied. Relative MUC1-SEC and MUC1-Y mRNAlevels were determined by qPCR and protein levels byWestern blotting. Induction of mucin mRNAs wasassayed in vitro. Immunohistochemistry was used forlocalization.RESULTS: Relative MUC1-SEC and MUC1-Y mRNA andprotein levels were significantly higher in LSG from SSpatients. These mRNAs were induced by cytokines.MUC1-SEC and MUC1-Y were detected in acini apicalregion of control LSGs, and significant cytoplasmicaccumulation was observed in acini of SS patients.MUC1-Y localized in acinar nuclei and cytoplasm ofinflammatory cells of LSG from SS patients. A strongpositive correlation was observed between cellularMUC1-SEC levels and glandular function determined byscintigraphy.CONCLUSIONS: We show for the first time thatMUC1-SEC and MUC1-Y are expressed in LSG of bothSS patients and non-SS sicca subjects. The observedoverexpression and aberrant localization of MUC1-SECand MUC1-Y and their induction by pro-inflammatorycytokines may favor the perpetuation of the inflammatoryenvironment that disrupts the salivary glandularhomeostasis in SS patients.Nota general

Artículo de publicación ISI

Autor: Sung Hsieh, Hsiao; - Castro, I.; - González, S.; - Aguilera, S.; - Smorodinsky, N. I.; - Quest, Andrew F. G.; - Bahamondes Lorca



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