In Vivo Evaluation of Complex Biogenic Silver Nanoparticle and Enoxaparin in Wound HealingReport as inadecuate

In Vivo Evaluation of Complex Biogenic Silver Nanoparticle and Enoxaparin in Wound Healing - Download this document for free, or read online. Document in PDF available to download.

Journal of Nanomaterials - Volume 2015 2015, Article ID 439820, 10 pages -

Research Article

Solid State Chemistry Lab. LQES, Chemistry Institute, Universidade Estadual de Campinas, CP 6154, 13083-970 Campinas, SP, Brazil

Nanobiolab, School of Pharmaceutical Sciences of Riberão Preto, Universidade de São Paulo, 14030-940 Ribeirão Preto, SP, Brazil

Biochemistry Department, Universidade Estadual de Campinas, CP 6109, 13083-970 Campinas, SP, Brazil

Faculdade Metrocamp, 13035-500 Campinas, SP, Brazil

Hospital Sobrapar, CP 6028, 13083-880 Campinas, SP, Brazil

School of Applied Sciences, Universidade Estadual de Campinas, CP 1068, 13484-350 Limeira, SP, Brazil

Received 10 December 2014; Revised 27 February 2015; Accepted 3 March 2015

Academic Editor: Jin-Ho Choy

Copyright © 2015 P. D. Marcato et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The burns treatment is difficult, uncomfortable for the patient, and expensive for health system. Due to antimicrobial properties of silver nanoparticles AgNP, these particles can avoid bacterial infection in wound and accelerate the wound healing. Furthermore, the complexation of AgNP with enoxaparin low molecular weight heparin may improve the healing process of lesions due to anti-inflammatory and angiogenic activity of enoxaparin Enox. The aim of this study was evaluated the activity and toxicity of biogenic AgNP and AgNP complexed with Enox in in vivo burn wound model. AgNP was produced by biosynthesis method using Fusarium oxysporum. AgNP 20–40 nm exhibited high stability due to protein capping around the particles that was confirmed by TEM, fluorescence spectroscopy, and FTIR. The wound contraction in in vivo model, after 28 days of treatment, was 55, 89, 91, and 95% for control, Enox, AgNP, and AgNP-Enox groups, respectively. No clear toxic effects in the biochemistry and hematological parameters were verified in all treated groups. However, in the AgNP-Enox group, a statistically significant increase in the urea levels was observed indicating increased proteolysis due to inflammation process. The results demonstrated that the complex AgNP-Enox is interesting for wound healing decreasing the time of lesions healing.

Author: P. D. Marcato, L. B. De Paula, P. S. Melo, I. R. Ferreira, A. B. A. Almeida, A. S. Torsoni, and O. L. Alves



Related documents