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BioMed Research International - Volume 2015 2015, Article ID 439808, 9 pages -

Research Article

ICREC Research Program, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, 08916 Badalona, Spain

IVECAT Group, Germans Trias i Pujol Health Science Research Institute, Can Ruti Campus, 08916 Badalona, Spain

Cardiology Service, Germans Trias i Pujol University Hospital, 08916 Badalona, Spain

Department of Medicine, UAB, 08916 Badalona, Spain

Nephrology Service, Germans Trias i Pujol University Hospital, 08916 Badalona, Spain

Received 25 November 2014; Revised 20 January 2015; Accepted 16 February 2015

Academic Editor: Betti Giusti

Copyright © 2015 Isaac Perea-Gil et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cell-based strategies to regenerate injured myocardial tissue have emerged over the past decade, but the optimum cell type is still under scrutiny. In this context, human adult epicardial fat surrounding the heart has been characterized as a reservoir of mesenchymal-like progenitor cells cardiac ATDPCs with potential clinical benefits. However, additional data on the possibility that these cells could trigger a deleterious immune response following implantation are needed. Thus, in the presented study, we took advantage of the well-established low immunogenicity of umbilical cord blood-derived mesenchymal stem cells UCBMSCs to comparatively assess the immunomodulatory properties of cardiac ATDPCs in an in vitro allostimulatory assay using allogeneic mature monocyte-derived dendritic cells MDDCs. Similar to UCBMSCs, increasing amounts of seeded cardiac ATDPCs suppressed the alloproliferation of T cells in a dose-dependent manner. Secretion of proinflammatory cytokines IL6, TNFα, and IFNγ was also specifically modulated by the different numbers of cardiac ATDPCs cocultured. In summary, we show that cardiac ATDPCs abrogate T cell alloproliferation upon stimulation with allogeneic mature MDDCs, suggesting that they could further regulate a possible harmful immune response in vivo. Additionally, UCBMSCs can be considered as valuable tools to preclinically predict the immunogenicity of prospective regenerative cells.





Autor: Isaac Perea-Gil, Marta Monguió-Tortajada, Carolina Gálvez-Montón, Antoni Bayes-Genis, Francesc E. Borràs, and Santiago Ro

Fuente: https://www.hindawi.com/



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