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Biological Procedures Online

, Volume 10, Issue 1, pp 83–89

Received: 13 February 2008Revised: 20 March 2008Accepted: 26 May 2008DOI: 10.1251-bpo146

Cite this article as: Lavigne, C., de Guignée, A. & Thierry, A.R. Biol. Proced. Online 2008 10: 83. doi:10.1251-bpo146

Abstract

In this paper, we describe a simple, rapid, specific, sensitive, and reliable method, the FICP method Fluorescence Immunoassay for Cellular Protein detection which is readily applicable to the detection of proteins directly on cells cultured in 96-well plates. In order to illustrate this method, we report on the detection of two different proteins, the cell cycle proteins cyclin D1 and p21CIP1-WAF1, in untreated and 2-cyclopenten-1-one treated breast cancer cells. When the FICP method was compared with Western blot procedure, FICP was found to be superior for many characteristics. By using this method, we were able to quantify biological effects of a specific compound on protein levels in non-lysed cells and perform statistical analysis. Therefore, we believe this screening assay could be very useful for detecting poorly expressed proteins and for drug development.

Indexing TermsFluoroimmunoassay Drug Evaluation  Download to read the full article text



Autor: Carole Lavigne - Armèle de Guignée - Alain R. Thierry

Fuente: https://link.springer.com/



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