Recent advances in freeze-fracture electron microscop: the replica immunolabeling techniqueReport as inadecuate

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Biological Procedures Online

, Volume 10, Issue 1, pp 9–19

Received: 18 October 2007Revised: 27 November 2007Accepted: 12 December 2007DOI: 10.1251-bpo138

Cite this article as: Robenek, H. & Severs, N.J. Biol. Proced. Online 2008 10: 9. doi:10.1251-bpo138


Freeze-fracture electron microscopy is a technique for examining the ultrastructure of rapidly frozen biological samples by transmission electron microscopy. Of a range of approaches to freeze-fracture cytochemistry that have been developed and tried the most successful is the technique termed freeze-fracture replica immunogold labeling FRIL. In this technique samples are frozen fractured and replicated with platinum-carbon as in standard freeze fracture and then carefully treated with sodium dodecylsulphate to remove all the biological material except a fine layer of molecules attached to the replica itself. Immunogold labeling of these molecules permits their distribution to be seen superimposed upon high resolution planar views of membrane structure. Examples of how this technique has contributed to our understanding of lipid droplet biogenesis and function are discussed.

Indexing termsFreeze Fracturing Microscopy, Immunoelectron Immunoǵold Techniques  Download to read the full article text

Author: Horst Robenek - Nicholas J. Severs


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