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Purinergic Signalling

, Volume 4, Issue 4, pp 331–338

First Online: 27 May 2008Received: 05 March 2008Accepted: 17 April 2008DOI: 10.1007-s11302-008-9108-0

Cite this article as: Greig, A.V.H., Linge, C. & Burnstock, G. Purinergic Signalling 2008 4: 331. doi:10.1007-s11302-008-9108-0

Abstract

We investigated the expression of P2X5, P2X7, P2Y1 and P2Y2 receptor subtypes in adult human anagen hair follicles and in relation to markers of proliferation proliferating cell nuclear antigen PCNA and Ki-67, keratinocyte differentiation involucrin and apoptosis anticaspase-3. Using immunohistochemistry, we showed that P2X5, P2Y1 and P2Y2 receptors were expressed in spatially distinct zones of the anagen hair follicle: P2Y1 receptors in the outer root sheath and bulb, P2X5 receptors in the inner and outer root sheaths and medulla and P2Y2 receptors in living cells at the edge of the cortex-medulla. P2X7 receptors were not expressed. Colocalisation experiments suggested different functional roles for these receptors: P2Y1 receptors were associated with bulb and outer root sheath keratinocyte proliferation, P2X5 receptors were associated with differentiation of cells of the medulla and inner root sheaths and P2Y2 receptors were associated with early differentiated cells in the cortex-medulla that contribute to the formation of the hair shaft. The therapeutic potential of purinergic agonists and antagonists for controlling hair growth is discussed.

KeywordsReceptors Purinergic Hair follicle Proliferation Differentiation AbbreviationsAAamino acids

ATPadenosine 5’-triphosphate

ADPadenosine 5’-diphosphate

DABdiaminobenzidine

NGSnormal goat serum

NHSnormal horse serum

IRSinner root sheath

ORSouter root sheath

PBSphosphate buffered saline

PCNAproliferating cell nuclear antigen

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Autor: Aina V. H. Greig - Claire Linge - Geoffrey Burnstock

Fuente: https://link.springer.com/







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