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Journal of Chemistry - Volume 2017 2017, Article ID 9191382, 6 pages - https:-doi.org-10.1155-2017-9191382

Research Article

Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China

State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China

College of Food Science, Northeast Agricultural University, Harbin 150030, China

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China

Correspondence should be addressed to Jia-Ying Xin

Received 17 October 2016; Accepted 4 January 2017; Published 16 February 2017

Academic Editor: Pasquale Longo

Copyright © 2017 Jia-Ying Xin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Methane monooxygenase MMO has been found in methanotrophic bacteria, which catalyzes the epoxidation of gaseous alkenes to their corresponding epoxides. The whole cell suspension of Methylosinus trichosporium IMV 3011 was used to produce epoxyethane from ethylene. The optimal reaction time and initial ethylene concentration for ethylene epoxidation have been described. The product epoxyethane is not further metabolized and accumulates extracellularly. Thus, exhaustion of reductant and the inhibition of toxic products make it difficult to accumulate epoxyethane continuously. In order to settle these problems, regeneration of cofactor NADH was performed in batch experiments with methane and methanol. The amount of epoxyethane formed before cosubstrate regeneration was between 0.8 and 1.0 nmol-50 mg cells in approximately 8 h. Combining data from 7 batch experiments, the total production of epoxyethane was 2.2 nmol. Production of epoxyethane was improved 4.6 nmol in 10% gas phase methane since methane acts as an abundant reductant for epoxidation. It was found that the maximum production of epoxyethane 6.6 nmol occurs with 3 mmol-L methanol. The passive effect of epoxyethane accumulation on epoxyethane production capacity of Methylosinus trichosporium IMV 3011 in batch experiments was studied. Removal of product was suggested to overcome the inhibition of epoxyethane production.

Autor: Jia-Ying Xin, Ning Xu, Sheng-Fu Ji, Yan Wang, and Chun-Gu Xia

Fuente: https://www.hindawi.com/


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