Differences in PfEMP1s recognized by antibodies from patients with uncomplicated or severe malariaReportar como inadecuado




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Malaria Journal

, 15:258

First Online: 05 May 2016Received: 27 October 2015Accepted: 15 April 2016DOI: 10.1186-s12936-016-1296-4

Cite this article as: Duffy, M.F., Noviyanti, R., Tsuboi, T. et al. Malar J 2016 15: 258. doi:10.1186-s12936-016-1296-4

Abstract

BackgroundPlasmodium falciparum erythrocyte membrane protein 1 PfEMP1 variants are encoded by var genes and mediate pathogenic cytoadhesion and antigenic variation in malaria. PfEMP1s can be broadly divided into three principal groups A, B and C and they contain conserved arrangements of functional domains called domain cassettes. Despite their tremendous diversity there is compelling evidence that a restricted subset of PfEMP1s is expressed in severe disease. In this study antibodies from patients with severe and uncomplicated malaria were compared for differences in reactivity with a range of PfEMP1s to determine whether antibodies to particular PfEMP1 domains were associated with severe or uncomplicated malaria.

MethodsParts of expressed var genes in a severe malaria patient were identified by RNAseq and several of these partial PfEMP1 domains were expressed together with others from laboratory isolates. Antibodies from Papuan patients to these parts of multiple PfEMP1 proteins were measured.

ResultsPatients with uncomplicated malaria were more likely to have antibodies that recognized PfEMP1 of Group C type and recognized a broader repertoire of group A and B PfEMP1s than patients with severe malaria.

ConclusionThese data suggest that exposure to a broad range of group A and B PfEMP1s is associated with protection from severe disease in Papua, Indonesia.

KeywordsSevere malaria var genes PfEMP1 Electronic supplementary materialThe online version of this article doi:10.1186-s12936-016-1296-4 contains supplementary material, which is available to authorized users.

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Autor: Michael F. Duffy - Rintis Noviyanti - Takafumi Tsuboi - Zhi-Ping Feng - Leily Trianty - Boni F. Sebayang - Eizo Takashima

Fuente: https://link.springer.com/







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