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Journal of Biology

, 5:7

First Online: 27 April 2006Received: 05 November 2005Revised: 21 March 2006Accepted: 22 March 2006DOI: 10.1186-jbiol35

Cite this article as: Davies, J.E., Huang, C., Proschel, C. et al. J Biol 2006 5: 7. doi:10.1186-jbiol35


BackgroundTransplantation of embryonic stem or neural progenitor cells is an attractive strategy for repair of the injured central nervous system. Transplantation of these cells alone to acute spinal cord injuries has not, however, resulted in robust axon regeneration beyond the sites of injury. This may be due to progenitors differentiating to cell types that support axon growth poorly and-or their inability to modify the inhibitory environment of adult central nervous system CNS injuries. We reasoned therefore that pre-differentiation of embryonic neural precursors to astrocytes, which are thought to support axon growth in the injured immature CNS, would be more beneficial for CNS repair.

ResultsTransplantation of astrocytes derived from embryonic glial-restricted precursors GRPs promoted robust axon growth and restoration of locomotor function after acute transection injuries of the adult rat spinal cord. Transplantation of GRP-derived astrocytes GDAs into dorsal column injuries promoted growth of over 60% of ascending dorsal column axons into the centers of the lesions, with 66% of these axons extending beyond the injury sites. Grid-walk analysis of GDA-transplanted rats with rubrospinal tract injuries revealed significant improvements in locomotor function. GDA transplantation also induced a striking realignment of injured tissue, suppressed initial scarring and rescued axotomized CNS neurons with cut axons from atrophy. In sharp contrast, undifferentiated GRPs failed to suppress scar formation or support axon growth and locomotor recovery.

ConclusionPre-differentiation of glial precursors into GDAs before transplantation into spinal cord injuries leads to significantly improved outcomes over precursor cell transplantation, providing both a novel strategy and a highly effective new cell type for repairing CNS injuries.

Electronic supplementary materialThe online version of this article doi:10.1186-jbiol35 contains supplementary material, which is available to authorized users.

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Autor: Jeannette E Davies - Carol Huang - Christoph Proschel - Mark Noble - Margot Mayer-Proschel - Stephen JA Davies


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