Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilizationReportar como inadecuado




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Theoretical Biology and Medical Modelling

, 2:25

First Online: 18 July 2005Received: 01 April 2005Accepted: 18 July 2005DOI: 10.1186-1742-4682-2-25

Cite this article as: Sills, E.S., Takeuchi, T., Tanaka, N. et al. Theor Biol Med Model 2005 2: 25. doi:10.1186-1742-4682-2-25

Abstract

BackgroundEmbryonic stem cells ESC are pluripotent cells obtained from the inner cell mass ICM of blastocysts derived from in vitro culture associated with reproductive endocrinology therapy. Human ESCs are regarded as highly significant since they retain the capacity to differentiate into any of approximately 200 unique cell types. Human ESC research is controversial because to acquire such cells, the ICM of human blastocysts must be manipulated in a way that renders embryos nonviable and unsuitable for transfer in utero. Techniques to yield competent ESCs with conservation of source blastocysts would satisfy many objections against ESC research, but at present such approaches remain largely untested.

Results and discussionWe contrast experimental culture of single blastomeres obtained by 1 non-destructive biopsy of embryos destined for transfer, and 2 isolation of karyotypically normal blastomeres from disaggregated -dead- embryos considered unsuitable for transfer, and evaluate these approaches with regard to production of ESCs. Pluripotency was confirmed by morphological criteria and by quantification of divergent homeodomain proteins specific to undifferentiated cell development. Following ESC isolation and identification, assessment was conducted according to a novel ESC grading system, also proposed here.

ConclusionThe role of reproductive endocrinology in ESC research remains paramount. In this report, we hypothesize new and expand on existing strategies having the potential to enhance human ESC isolation, identification and in vitro maintenance.

Electronic supplementary materialThe online version of this article doi:10.1186-1742-4682-2-25 contains supplementary material, which is available to authorized users.

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Autor: Eric Scott Sills - Takumi Takeuchi - Noriko Tanaka - Queenie V Neri - Gianpiero D Palermo

Fuente: https://link.springer.com/







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