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Biological Procedures Online

, Volume 5, Issue 1, pp 153–161

Received: 28 April 2003Revised: 09 June 2003Accepted: 09 June 2003DOI: 10.1251-bpo57

Cite this article as: Maiato, H., Sunkel, C.E. & Earnshaw, W.C. Biol. Proced. Online 2003 5: 153. doi:10.1251-bpo57

Abstract

Here we describe a detailed methodology to study the function of genes whose products function during mitosis by dsRNA-mediated interference RNAi in cultured cells ofDrosophila melanogaster. This procedure is particularly useful for the analysis of genes for which genetic mutations are not available or for the dissection of complicated phenotypes derived from the analysis of such mutants. With the advent of whole genome sequencing it is expected that RNAi-based screenings will be one method of choice for the identification and study of novel genes involved in particular cellular processes. In this paper we focused particularly on the procedures for the proper phenotypic analysis of cells after RNAi-mediated depletion of proteins required for mitosis, the process by which the genetic information is segregated equally between daughter cells. We use RNAi of the microtubule-associated protein MAST-Orbit as an example for the usefulness of the technique.

Indexing termsRNAi Mitosis MAST Orbit Microtubule MAP Kinetochore Spindle Drosophila Published: June 15, 2003

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Autor: Helder Maiato - Claudio E. Sunkel - William C. Earnshaw

Fuente: https://link.springer.com/







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