An Exploratory Study on the Development of an Animal Model of Acute Pancreatitis Following Nicotine ExposureReportar como inadecuado

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Tobacco Induced Diseases

, 1:213

First Online: 15 September 2003DOI: 10.1186-1617-9625-1-3-213

Cite this article as: Chowdhury, P. Tob. Induced Dis. 2003 1: 213. doi:10.1186-1617-9625-1-3-213


AbstractCigarette smoking is known to be a major risk factor for pancreatic cancer and pancreatitis is believed to be a predisposed condition for pancreatic cancer. As of this date, there is no established experimental animal model to conduct detailed studies on these two deadly diseases. Our aim is to establish a rodent model by which we can systematically study the pathogenesis of pancreatitis and pancreatic cancer.

MethodsAdult Male Sprague Dawley rats were exposed to graded doses of nicotine by various routes for periods of three to 16 weeks. Blood samples were measured for hormonal and metabolic parameters. The pancreas was evaluated for histopathological changes and its function was assessed in isolated pancreatic acini upon stimulation with cholecystokinin CCK or carbachol Cch. The pancreatic tissue was evaluated further for oncogene expression.

ResultsBody weight, food and fluid intakes, plasma glucose and insulin levels were significantly reduced in animals with nicotine exposure when compared to control. However, CCK and gastrin levels in the blood were significantly elevated. Pancreatic function was decreased significantly with no alteration in CCK receptor binding. Pancreatic histology revealed vacuolation, swelling, cellular pyknosis and karyorrhexis. Mutant oncogene, H-ras, was overexpressed in nicotine-treated pancreatic tissue.

Summary and conclusionThe results suggest that alterations in metabolic, hormonal and pathologic parameters following nicotine-treatment appear consistent with diagnostic criteria of human pancreatitis. It is proposed that rats could be considered as a potential animal model to study the pathogenesis of pancreatitis.

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Autor: P Chowdhury


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