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Biological Procedures Online

, Volume 5, Issue 1, pp 228–237

Received: 26 August 2003Revised: 19 November 2003Accepted: 20 November 2003DOI: 10.1251-bpo66

Cite this article as: Pridgeon, J.W., Geetha, T. & Wooten, M.W. Biol. Proced. Online 2003 5: 228. doi:10.1251-bpo66


The UBA domain is a conserved sequence motif among polyubiquitin binding proteins. For the first time, we demonstrate a systematic, high throughput approach to identification of UBA domain-interacting proteins from a proteome-wide perspective. Using the rabbit reticulocyte lysatein vitro expression cloning system, we have successfully identified eleven proteins that interact with p62’s UBA domain, and the majority of the eleven proteins are associated with neurodegenerative disorders, such as Alzheimer’s disease. Therefore, p62 may play a novel regulatory role through its UBA domain. Our approach provides an easy route to the characterization of UBA domain interacting proteins and its application will unfold the important roles that the UBA domain plays.

Indexing termsUbiquitin Alzheimer Disease Published: December 12, 2003.

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Autor: Julia W. Pridgeon - Thangiah Geetha - Marie W. Wooten

Fuente: https://link.springer.com/

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