Shotgun phage display — Selection for bacterial receptins or other exported proteinsReport as inadecuate




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Biological Procedures Online

, 5:123

Received: 11 February 2003Revised: 31 March 2003Accepted: 08 April 2003DOI: 10.1251-bpo54

Cite this article as: Jacobsson, K., Rosander, A., Bjerketorp, J. et al. Biol. Proced. Online 2003 5: 123. doi:10.1251-bpo54

Abstract

Shotgun phage display cloning involves construction of libraries from randomly fragmented bacterial chromosomal DNA, cloned genes, or eukaryotic cDNAs, into a phagemid vector. The library obtained consists of phages expressing polypeptides corresponding to all genes encoded by the organism, or overlapping peptides derived from the cloned gene. From such a library, polypeptides with affinity for another molecule can be isolated by affinity selection, panning. The technique can be used to identify bacterial receptins and identification of their minimal binding domain, and but also to identify epitopes recognised by antibodies. In addition, after modification of the phagemid vector, the technique has also been used to identify bacterial extracytoplasmic proteins.

Indexing termsPeptide Library Staphylococcus aureus Published: May 1, 2003

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Author: Karin Jacobsson - Anna Rosander - Joakim Bjerketorp - Lars Frykberg

Source: https://link.springer.com/







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