Isolation, growth and identification of colony-forming cells with erythroid, myeloid, dendritic cell and NK-cell potential from human fetal liverReportar como inadecuado




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Biological Procedures Online

, Volume 4, Issue 1, pp 10–23

Received: 16 April 2002Accepted: 27 May 2002DOI: 10.1251-bpo29

Cite this article as: Muench, M.O., Suskind, D.L. & Bárcena, A. Biol Proced Online 2002 4: 10. doi:10.1251-bpo29

Abstract

The study of hematopoietic stem cells HSCs and the process by which they differentiate into committed progenitors has been hampered by the lack of in vitro clonal assays that can support erythroid, myeloid and lymphoid differentiation. We describe a method for the isolation from human fetal liver of highly purified candidate HSCs and progenitors based on the phenotypes CD38CD34 and CD38CD34, respectively. We also describe a method for the growth of colony-forming cells CFCs from these cell populations, under defined culture conditions, that supports the differentiation of erythroid, CD14-CD15 myeloid, CD1a dendritic cell and CD56 NK cell lineages. Flow cytometric analyses of individual colonies demonstrate that CFCs with erythroid, myeloid and lymphoid potential are distributed among both the CD38 and CD38 populations of CD34 progenitors.

Indexing termsfetal tissue hematopoietic stem cells cell differentiation natural killer cells dendritic cells Published: June 11, 2002.

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Autor: Marcus O. Muench - David L. Suskind - Alicia Bárcena

Fuente: https://link.springer.com/







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