Mouse aortic ring assay: A new approach of the molecular genetics of angiogenesisReport as inadecuate

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Biological Procedures Online

, 4:24

Received: 29 May 2002Revised: 25 June 2002Accepted: 17 July 2002DOI: 10.1251-bpo30

Cite this article as: Masson, V., Devy, L., Grignet-Debrus, C. et al. Biol Proced Online 2002 4: 24. doi:10.1251-bpo30


Angiogenesis, a key step in many physiological and pathological processes, involves proteolysis of the extracellular matrix. To study the role of two enzymatic families, serine-proteases and matrix metalloproteases in angiogenesis, we have adapted to the mouse, the aortic ring assay initially developed in the rat. The use of deficient mice allowed us to demonstrate that PAI-1 is essential for angiogenesis while the absence of an MMP, MMP-11, did not affect vessel sprouting. We report here that this model is attractive to elucidate the cellular and molecular mechanisms of angiogenesis, to identify, characterise or screen -pro- or anti-angiogenic agents that could be used for the treatment of angiogenesis-dependent diseases. Approaches include using recombinant proteins, synthetic molecules and adenovirus-mediated gene transfer.

Indexing termsAngiogenesis factor endothelium transgenic mice recombinant adenovirus Published: October 28, 2002

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Author: Véronique Masson - Laetitia Devy - Christine Grignet-Debrus - Sarah Bernt - Khalid Bajou - Silvia Blacher - Guy Roland - Y


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