Acid glycohydrolases in rat spermatocytes, spermatids and spermatozoa: enzyme activities, biosynthesis and immunolocalizationReport as inadecuate




Acid glycohydrolases in rat spermatocytes, spermatids and spermatozoa: enzyme activities, biosynthesis and immunolocalization - Download this document for free, or read online. Document in PDF available to download.

Biological Procedures Online

, Volume 3, Issue 1, pp 35–42

Received: 19 April 2001Accepted: 19 November 2001DOI: 10.1251-bpo21

Cite this article as: Abou-Haila, A. & Tulsiani, D.R.P. Biol Proced Online 2001 3: 35. doi:10.1251-bpo21

Abstract

Mammalian sperm acrosomes contain several glycohydrolases that are thought to aid in the dispersion and digestion of vestments surrounding the egg. In this study, we have used multiple approaches to examine the origin of acrosome-associated glycohdyrdolases. Mixed spermatogenic cells, prepared from rat testis, were separated by unit gravity sedimentation. The purified germ cells spermatocytes SC, round spermatids RS, and elongated-condensed spermatids E-CS contained several glycohydrolase activities. Metabolic labeling in the cell culture, immunoprecipitation, and autoradiographic approaches revealed that β-D-galactosidase was synthesized in SC and RS in 88-90 kDa forms which undergo processing in a cell-specific manner. Immunohistochemical approaches demonstrated that the enzyme was localized in Golgi membranes-vesicles, and lysosome-like structures in SC and RS, and forming-formed acrosome of E-CS.

Indexing termsSperm acrosome male germ cells β-D-galactosidase acrosmal glycohydrolases mammalian fertilization AbbreviationsMEMminimum essential medium

BSAbovine serum albumin

PBSphosphate buffered saline

SDSsodium dodecyl sulphate

PAGEpolyacrylamide gel electrophoresis

PNPp-nitrophenyl

EKRBenriched Krebs-Ringer bicarbonate

Published December 3, 2001

Download to read the full article text



Author: Aida Abou-Haila - Daulat R. P. Tulsiani

Source: https://link.springer.com/







Related documents