Methods for microbial DNA extraction from soil for PCR amplificationReport as inadecuate

Methods for microbial DNA extraction from soil for PCR amplification - Download this document for free, or read online. Document in PDF available to download.

Biological Procedures Online

, Volume 1, Issue 1, pp 40–47

DOI: 10.1251-bpo6

Cite this article as: Yeates, C., Gillings, M.R., Davison, A.D. et al. Biol Proced Online 1998 1: 40. doi:10.1251-bpo6


Amplification of DNA from soil is often inhibited by co-purified contaminants. A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types 1. DNA is also suitable for PCR amplification using various DNA targets. DNA was extracted from 100g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and isopropanol precipitation. This method was compared to other DNA extraction methods with regard to DNA purity and size.

Download to read the full article text

Author: C. Yeates - M. R. Gillings - A. D. Davison - N. Altavilla - D. A. Veal


Related documents