Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210Reportar como inadecuado

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Oxidative Medicine and Cellular Longevity - Volume 2017 2017, Article ID 3565613, 11 pages -

Research ArticleDepartment of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin, China

Correspondence should be addressed to Bin Liu

Received 4 November 2016; Revised 12 January 2017; Accepted 9 February 2017; Published 7 March 2017

Academic Editor: Jaideep Banerjee

Copyright © 2017 Tianyi Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Oxidative stress induces endothelial cell apoptosis and promotes atherosclerosis development. MicroRNA-210 miR-210 is linked with apoptosis in different cell types. This study aimed to investigate the role of miR-210 in human umbilical vein endothelial cells HUVECs under oxidative stress and to determine the underlying mechanism. HUVECs were treated with different concentrations of hydrogen peroxide H2O2, and cell viability was evaluated using the 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assay and ATP assay. To evaluate the role of miR-210 in H2O2-mediated apoptosis, gain-and-loss-of-function approaches were used, and the effects on apoptosis and reactive oxygen species ROS level were assayed using flow cytometry. Moreover, miR-210 expression was detected by quantitative reverse transcriptase polymerase chain reaction qRT-PCR, and expression of the following apoptosis-related genes was assessed by qRT-PCR and Western blot at the RNA and protein level, respectively: caspase-8-associated protein 2 CASP8AP2, caspase-8, and caspase-3. The results showed that H2O2 induced apoptosis in HUVECs in a dose-dependent manner and increased miR-210 expression. Overexpression of miR-210 inhibited apoptosis and reduced ROS level in HUVECs treated with H2O2. Furthermore, miR-210 downregulated CASP8AP2 and related downstream caspases at protein level. Thus, under oxidative stress, miR-210 has a prosurvival and antiapoptotic effect on HUVECs by reducing ROS generation and downregulating the CASP8AP2 pathway.

Autor: Tianyi Li, Xianjing Song, Jichang Zhang, Lei Zhao, Yongfeng Shi, Zhibo Li, Jia Liu, Ning Liu, Youyou Yan, Yanlong Xiao, 



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