BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal DamageReport as inadecuate




BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2014 2014, Article ID 356415, 11 pages -

Research Article

Center for Hearing and Communication Research, Karolinska University Hospital, 171 76 Stockholm, Sweden

Department of Clinical Sciences, Intervention and Technology CLINTEC, Section of Otorhinolaryngology, Karolinska Institutet, Karolinska University Hospital, 171 76 Stockholm, Sweden

Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Department of Ophthalmology, Institution of Clinical Sciences in Lund, Lund University, 221 84 Lund, Sweden

Departments of Chemistry, Materials Science and Engineering, and Medicine and Institute for Bionanotechnology in Medicine, Northwestern University, Chicago, IL 60208, USA

Division of Otorhinolaryngology, Linköping University Hospital, 581 85 Linköping, Sweden

Division of Otorhinolaryngology, Department of Clinical and Experimental Medicine, University of Linköping, 581 85 Linköping, Sweden

Received 21 February 2014; Revised 2 May 2014; Accepted 29 July 2014; Published 26 August 2014

Academic Editor: Jonathan Gale

Copyright © 2014 Yu Jiao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives. To study possible nerve regeneration of a damaged auditory nerve by the use of stem cell transplantation. Methods. We transplanted HNPCs to the rat AN trunk by the internal auditory meatus IAM. Furthermore, we studied if addition of BDNF affects survival and phenotypic differentiation of the grafted HNPCs. A bioactive nanofiber gel PA gel, in selected groups mixed with BDNF, was applied close to the implanted cells. Before transplantation, all rats had been deafened by a round window niche application of β-bungarotoxin. This neurotoxin causes a selective toxic destruction of the AN while keeping the hair cells intact. Results. Overall, HNPCs survived well for up to six weeks in all groups. However, transplants receiving the BDNF-containing PA gel demonstrated significantly higher numbers of HNPCs and neuronal differentiation. At six weeks, a majority of the HNPCs had migrated into the brain stem and differentiated. Differentiated human cells as well as neurites were observed in the vicinity of the cochlear nucleus. Conclusion. Our results indicate that human neural precursor cells HNPC integration with host tissue benefits from additional brain derived neurotrophic factor BDNF treatment and that these cells appear to be good candidates for further regenerative studies on the auditory nerve AN.





Author: Yu Jiao, Björn Palmgren, Ekaterina Novozhilova, Ulrica Englund Johansson, Anne L. Spieles-Engemann, Ajay Kale, Samuel I. St

Source: https://www.hindawi.com/



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