Pathogenic potential and growth kinetics of Muko virus in mice and human-derived cellsReport as inadecuate

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Tropical Medicine and Health

, 44:31

First Online: 11 October 2016Received: 09 June 2016Accepted: 17 August 2016DOI: 10.1186-s41182-016-0032-7

Cite this article as: Ulanday, G.E.L., Shimada, S., Myat, N.T.M. et al. Trop Med Health 2016 44: 31. doi:10.1186-s41182-016-0032-7


BackgroundTicks have been long known as vectors of various pathogens, some of which can cause high fatality rates among infected individuals. Our enhanced tick surveillance around Nagasaki, Japan, led to the isolation and identification of a new strain of a recently identified Orbivirus, Muko virus MUV. The orbiviruses have a wide host range, including humans, and is related to a spectrum of clinical outcomes. However, the zoonotic potential of some members of the genus, although reported, were not clearly elucidated. Hence, it is imperative to characterize newly isolated orbiviruses and investigate its ability to endanger public health.

MethodsIn this study, we explored the in vivo pathogenicity of a newly isolated MUV strain MUV-Hay using a mouse model and demonstrated its growth kinetics in human-derived cells.

ResultsOur results showed the ability of MUV-Hay to propagate in human neuronal and renal cells with some cytopathic effect. Furthermore, intracerebral inoculation of our new isolate caused high mortality in adult A129 mice.

ConclusionOur study provided a first step to experimentally test the hypothesis, that MUV can replicate and produce cytopathic effect in human cells and demonstrate virulence in adult mice.

KeywordsMuko virus Tick-borne virus Mouse model Human-derived cells AbbreviationsMUVMuko virus

TBETick-borne encephalitis

TBEVTick-borne encephalitis virus

CCHFVCrimean-Congo hemorrhagic fever virus

SFTSSevere fever with thrombocytopenia syndrome

SFTSVSevere fever with thrombocytopenia syndrome virus

TFLVTofla virus

IFNAR KOInterferon-α-β receptor knockout

NGSNext-generation sequencing

EMEMEagle’s minimum essential medium

FBSFetal bovine serum


pfuPlaque-forming unit

CPECytopathic effect

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Author: Gianne Eduard L. Ulanday - Satoshi Shimada - Ngwe Tun Mya Myat - Takeshi Nabeshima - Kouichi Morita - Daisuke Hayasaka


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