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International Journal of Molecular ImagingVolume 2013 2013, Article ID 426961, 13 pages

Research Article

Optical Imaging Laboratory, Cluster in Biomedicine CBM scrl, 34149 Trieste, Italy

Department of Cardiology, University of Ferrara, Salvatore Maugeri Foundation, IRCCS, 44100 Ferrara, Italy

VisualSonics Inc., Toronto, ON, Canada M4N 3N1

Centro Interdipartimentale di Medicina Rigenerativa, University of Udine, 33100 Udine, Italy

Ephoran Multi-Imaging Solutions, 10010 Colleretto Giacosa, Torino, Italy

Received 12 October 2012; Accepted 23 November 2012

Academic Editor: Adriaan A. Lammertsma

Copyright © 2013 Chiara Garrovo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Stem cells are characterized by the ability to renew themselves and to differentiate into specialized cell types, while stem cell therapy is believed to treat a number of different human diseases through either cell regeneration or paracrine effects. Herein, an in vivo and ex vivo near infrared time domain NIR TD optical imaging study was undertaken to evaluate the migratory ability of murine adipose tissue-derived multipotent adult stem cells mAT-MASC after intramuscular injection in mice. In vivo NIR TD optical imaging data analysis showed a migration of DiD-labelled mAT-MASC in the leg opposite the injection site, which was confirmed by a fibered confocal microendoscopy system. Ex vivo NIR TD optical imaging results showed a systemic distribution of labelled cells. Considering a potential microenvironmental contamination, a cross-validation study by multimodality approaches was followed: mAT-MASC were isolated from male mice expressing constitutively eGFP, which was detectable using techniques of immunofluorescence and qPCR. Y-chromosome positive cells, injected into wild-type female recipients, were detected by FISH. Cross-validation confirmed the data obtained by in vivo-ex vivo TD optical imaging analysis. In summary, our data demonstrates the usefulness of NIR TD optical imaging in tracking delivered cells, giving insights into the migratory properties of the injected cells.

Autor: Chiara Garrovo, Natascha Bergamin, Dave Bates, Daniela Cesselli, Antonio Paolo Beltrami, Andrea Lorenzon, Roberto Ferrari, 



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