Phospholipase C gamma and ERK1-2 Mitogen Activated Kinase Pathways are differentially modulated by Trypanosoma cruzi during tissue invasion in human placentaReportar como inadecuado




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Resumen

Chagas‘ disease is caused by the haemophlagelated protozoan Trypanosoma cruzi T. cruzi. During congenitaltransmission the parasite breaks down the placental barrier, however studies about the physiopathologyof this process are scarce. Different signal transduction pathways are involved during cellinvasion of the parasite. However, the possible role of those processes during tissue infection has notbeen studied. In the present study we analyzed the modulation of two signal transduction pathways,PLC-c and ERK1-2 MAPK, during ex vivo infection of human placental chorionic villi explants.Chorionic villi from healthy woman placentas were incubated in the presence or absence of 105 or 106T. cruzi trypomastigotes DM28c strain with or without specific inhibitors for each pathway. Effectiveinfection was tested determining parasite DNA by PCR. The activation of PLC-c and ERK1-2 MAPK signalingpathways was determined by western blotting and immunofluorescence.The low concentration of T. cruzi trypomastigotes activates both signaling pathways; however, the highconcentration of parasite induces a modest activation of the PLC-c pathway and impairs the ERK1-2MAPK pathway activation. Interestingly, inhibition of any of those signaling pathways did not preventparasite infection, as it was previously shown in cell cultures. We conclude that both signal transductionpathways are modulated during ex vivo T. cruzi infection of human placental chorionic villi explants.Nota general

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Autor: Castillo, Christian; - Villarroel, Arturo; - Duaso, Juan; - Cabrera Vallejos, Gonzalo; - Galanti Garrone, Norbel; - Maya, Juan Di

Fuente: http://repositorio.uchile.cl/



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