Shikonin Attenuates Concanavalin A-Induced Acute Liver Injury in Mice via Inhibition of the JNK PathwayReportar como inadecuado




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Mediators of Inflammation - Volume 2016 2016, Article ID 2748367, 14 pages -

Research Article

Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China

The First Clinical Medical College, Nanjing Medical University, Nanjing 210029, China

The First Affiliated Hospital of Soochow University, Suzhou 215006, China

Received 29 December 2015; Accepted 21 March 2016

Academic Editor: Anshu Agrawal

Copyright © 2016 Tong Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Shikonin possesses anti-inflammatory effects. However, its function in concanavalin A-induced acute liver injury remains uncertain. The aim of the present study was to investigate the functions of shikonin and its mechanism of protection on ConA-induced acute liver injury. Materials and Methods. Balb-C mice were exposed to ConA 20 mg-kg via tail vein injection to establish acute liver injury; shikonin 7.5 mg-kg and 12.5 mg-kg was intraperitoneally administered 2 h before the ConA injection. The serum liver enzyme levels and the inflammatory cytokine levels were determined at 3, 6, and 24 h after ConA injection. Results. After the injection of ConA, inflammatory cytokines IL-1β, TNF-α, and IFN-γ were significantly increased. Shikonin significantly ameliorated liver injury and histopathological changes and suppressed the release of inflammatory cytokines. The expressions of Bcl-2 and Bax were markedly affected by shikonin pretreatment. LC3, Beclin-1, and p-JNK expression levels were decreased in the shikonin-pretreated groups compared with the ConA-treated groups. Shikonin attenuated ConA-induced liver injury by reducing apoptosis and autophagy through the inhibition of the JNK pathway. Conclusion. Our results indicated that shikonin pretreatment attenuates ConA-induced acute liver injury by inhibiting apoptosis and autophagy through the suppression of the JNK pathway.





Autor: Tong Liu, Yujing Xia, Jingjing Li, Sainan Li, Jiao Feng, Liwei Wu, Rong Zhang, Shizan Xu, Keran Cheng, Yuqing Zhou, Shun

Fuente: https://www.hindawi.com/



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