Dissecting subsecond cadherin bound states reveals an efficient way for cells to achieve ultrafast probing of their environment.Reportar como inadecuado




Dissecting subsecond cadherin bound states reveals an efficient way for cells to achieve ultrafast probing of their environment. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 LAI - Adhésion et Inflammation 2 Department of Chemical and Biochemical Engineering

Abstract : Cells continuously probe their environment with membrane receptors, achieving subsecond adaptation of their behaviour Diez, G., Gerisch, G., Anderson, K., M?r-Taubenberger, A. and Bretschneider, T. 2006 Subsecond reorganization of the actin network in cell motility and chemotaxis. Proc. Natl. Acad. Sci. USA 102, 7601-7606, Shamri, R., Grabovsky, V., Gauguet, J.M., Feigelson, S., Manevich, E., Kolanus, W., Robinson, M.K., Staunton, D.E., von Andrian, U.H. and Alon, R. 2005 Lymphocyte arrest requires instantaneous induction of an extended LFA-1 conformation mediated by endothelium-bound chemokines. Nat. Immunol. 6, 497-606, Jiang, G., Huang, A.H., Cai, Y., Tanase, M. and Sheetz, M.P. 2006 Rigidity sensing at the leading edge through alphaVbeta3 integrins and RPTPalpha. Biophys. J. 90, 1804-2006. Recently, several receptors, including cadherins, were found to bind ligands with a lifetime of order of one second. Here we show at the single molecule level that homotypic C-cadherin association involves transient intermediates lasting less than a few tens of milliseconds. Further, these intermediates transitionned towards more stable states with a kinetic rate displaying exponential decrease with piconewton forces. These features enable cells to detect ligands or measure surrounding mechanical behaviour within a fraction of a second, much more rapidly than was previously thought.

Keywords : flow chamber binding kinetics molecular forces cadherins





Autor: Anne Pierres - Anil Prakasam - Dominique Touchard - Anne-Marie Benoliel - Pierre Bongrand - Deborah Leckband -

Fuente: https://hal.archives-ouvertes.fr/



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