In vivo quantification of quantum dot systemic transport in C57BL-6 hairless mice following skin application post-ultraviolet radiationReportar como inadecuado

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Particle and Fibre Toxicology

, 14:12

First Online: 14 April 2017Received: 02 December 2016Accepted: 27 March 2017DOI: 10.1186-s12989-017-0191-7

Cite this article as: Jatana, S., Palmer, B.C., Phelan, S.J. et al. Part Fibre Toxicol 2017 14: 12. doi:10.1186-s12989-017-0191-7


BackgroundPrevious work has demonstrated size, surface charge and skin barrier dependent penetration of nanoparticles into the viable layers of mouse skin. The goal of this work was to characterize the tissue distribution and mechanism of transport of nanoparticles beyond skin, with and without Ultraviolet Radiation UVR induced skin barrier disruption. Atomic absorption spectroscopy AAS, flow cytometry and confocal microscopy were used to examine the effect of UVR dose 180 and 360 mJ-cm UVB on the skin penetration and systemic distribution of quantum dot QD nanoparticles topically applied at different time-points post UVR using a hairless C57BL-6 mouse model.

ResultsResults indicate that QDs can penetrate mouse skin, regardless of UVR exposure, as evidenced by the increased cadmium in the local lymph nodes of all QD treated mice. The average % recovery for all treatment groups was 69.68% with ~66.84% of the applied dose recovered from the skin both epicutaneous and intracutaneous. An average of 0.024% of the applied dose was recovered from the lymph nodes across various treatment groups. When QDs are applied 4 days post UV irradiation, at the peak of the skin barrier defect and LC migration to the local lymph node, there is an increased cellular presence of QD in the lymph node; however, AAS analysis of local lymph nodes display no difference in cadmium levels due to UVR treatment.

ConclusionsOur data suggests that Langerhans cells LCs can engulf QDs in skin, but transport to the lymph node may occur by both cellular dendritic and macrophage and non-cellular mechanisms. It is interesting that these specific nanoparticles were retained in skin similarly regardless of UVR barrier disruption, but the observed skin immune cell interaction with nanoparticles suggest a potential for immunomodulation, which we are currently examining in a murine model of skin allergy.

KeywordsQuantum dots Atomic absorption spectroscopy Distal organ analysis Skin dendritic cells AbbreviationsAASAtomic absorption spectroscopy


CNTCarbon nanotubes

CPDCyclobutane pyrimidine dimers


ICP-MSInductively coupled plasma mass spectrometry

LCLangerhans cells


PEGPolyethylene glycol

QDQuantum dot

ROSReactive oxygen species

TEWLTransepidermal water loss

UVBUltraviolet Radiation B

UVRUltraviolet Radiation

Electronic supplementary materialThe online version of this article doi:10.1186-s12989-017-0191-7 contains supplementary material, which is available to authorized users.

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Autor: Samreen Jatana - Brian C. Palmer - Sarah J. Phelan - Robert Gelein - Lisa A. DeLouise


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