Human MMP28 expression is unresponsive to inflammatory stimuli and does not correlate to the grade of intervertebral disc degenerationReportar como inadecuado

Human MMP28 expression is unresponsive to inflammatory stimuli and does not correlate to the grade of intervertebral disc degeneration - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Negative Results in BioMedicine

, 10:9

First Online: 29 July 2011Received: 01 April 2011Accepted: 29 July 2011DOI: 10.1186-1477-5751-10-9

Cite this article as: Klawitter, M., Quero, L., Bertolo, A. et al. J Negat Results BioMed 2011 10: 9. doi:10.1186-1477-5751-10-9


BackgroundMMP28 epilysin is a recently discovered member of the MMP matrix metalloproteinase family that is, amongst others, expressed in osteoarthritic cartilage and intervertebral disc IVD tissue. In this study the hypothesis that increased expression of MMP28 correlates with higher grades of degeneration and is stimulated by the presence of proinflammatory molecules was tested. Gene expression levels of MMP28 were investigated in traumatic and degenerative human IVD tissue and correlated to the type of disease and the degree of degeneration Thompson grade. Quantification of MMP28 gene expression in human IVD tissue or in isolated cells after stimulation with the inflammatory mediators lipopolysaccharide LPS, interleukin IL-1β, tumor necrosis factor TNF-α or the histondeacetylase inhibitor trichostatin A was performed by real-time RT PCR.

ResultsWhile MMP28 expression was increased in individual cases with trauma or disc degeneration, there was no significant correlation between the grade of disease and MMP28 expression. Stimulation with LPS, IL-1β, TNF-α or trichostatin A did not alter MMP28 gene expression at any investigated time point or any concentration.

ConclusionsOur results demonstrate that gene expression of MMP28 in the IVD is not regulated by inflammatory mechanisms, is donor-dependent and cannot be positively or negatively linked to the grade of degeneration and only weakly to the occurrence of trauma. New hypotheses and future studies are needed to find the role of MMP28 in the intervertebral disc.

KeywordsMMP28 Epilysin Matrix metalloproteinase Intervertebral disc Inflammation AbbreviationsHDAChistondeacetylase


IVDintervertebral disc

MMPmatrix metalloproteinase

TNF-αtumor necrosis factor-α.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-5751-10-9 contains supplementary material, which is available to authorized users.

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Autor: Marina Klawitter - Lilian Quero - Alessando Bertolo - Marco Mehr - Jivko Stoyanov - Andreas G Nerlich - Juergen Klasen - N


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