Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1α and HIF2α in rat oviduct and endometriumReportar como inadecuado




Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1α and HIF2α in rat oviduct and endometrium - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Molecular Histology

, Volume 42, Issue 4, pp 341–354

First Online: 06 July 2011Received: 18 April 2011Accepted: 19 June 2011DOI: 10.1007-s10735-011-9338-2

Cite this article as: Gillies, R.M., Robinson, S.P., McPhail, L.D. et al. J Mol Hist 2011 42: 341. doi:10.1007-s10735-011-9338-2

Abstract

Hypoxia is thought to be critical in regulating physiological processes within the female reproductive system, including ovulation, composition of the fluid in the oviductal-uterine lumens and ovarian follicle development. This study examined the localisation of exogenous pimonidazole and endogenous hypoxia inducible factor 1α and 2α HIF1α -2α, glucose transporter type 1 GLUT1 and carbonic anhydrase 9 CAIX hypoxia-related antigens within the oviduct and uterus of the rat reproductive tract. The extent to which each endogenous antigen co-compartmentalised with pimonidazole was also assessed. Female Wistar Furth rats n = 10 were injected intraperitoneally with pimonidazole 60 mg-kg 1 h prior to death. Reproductive tissues were removed immediately following death and fixed in 4% paraformaldehyde before being embedded in paraffin. Serial sections were cut 6–7 μm thick and antigens of interest identified using standard immunohistochemical procedures. The mucosal epithelia of the ampulla, isthmus and uterus were immunopositive for pimonidazole in most sections. Co-compartmentalisation of pimonidazole with HIF1α was only expressed in the mucosa of the uterus whilst co-compartmentalisation with HIF2α was observed in the mucosa of the ampulla, isthmus and uterus. Both GLUT1 and CAIX were co-compartmentalised with pimonidazole in mucosa of the isthmus and uterus. This study confirms that mucosal regions of the rat oviduct and uterus frequently experience severe hypoxia and there are compartment specific variations in expression of endogenous hypoxia-related antigens, including the HIF isoforms. The latter observation may relate to target gene specificity of HIF isoforms or perhaps HIF2α’s responsiveness to non-hypoxic stimuli such as hypoglycaemia independently of HIF1α.

KeywordsOviduct Uterus Pimonidazole Hypoxia GLUT Carbonic anhydrase  Download fulltext PDF



Autor: Robert M. Gillies - Simon P. Robinson - Lesley D. McPhail - Nicholas D. Carter - Joanne F. Murray

Fuente: https://link.springer.com/







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