New physiological activities of myosuppressin, sulfakinin and NVP-like peptide in Zophobas atratus beetleReport as inadecuate

New physiological activities of myosuppressin, sulfakinin and NVP-like peptide in Zophobas atratus beetle - Download this document for free, or read online. Document in PDF available to download.

Journal of Comparative Physiology B

, Volume 181, Issue 6, pp 721–730

First Online: 16 March 2011Received: 05 October 2010Revised: 17 February 2011Accepted: 23 February 2011DOI: 10.1007-s00360-011-0563-5

Cite this article as: Marciniak, P., Kuczer, M. & Rosinski, G. J Comp Physiol B 2011 181: 721. doi:10.1007-s00360-011-0563-5


Three neuropeptides Zopat-MS-2 pEDVDHVFLRFa, Zopat-SK-1 pETSDDYGHLRFa and Zopat-NVPL-4trunc. GRWGGFA, recently isolated from the neuroendocrine system of the Zophobas atratus beetle, were tested for their myotropic and hyperglycaemic activities in this species. These peptides exerted differentiated dose-dependent and tissue specific physiological effects. Zopat-MS-2 inhibited contractions of the isolated heart, ejaculatory duct, oviduct and hindgut of adult beetles and induced bimodal effects in the heart contractile activity of pupae in vivo. It also increased the haemolymph free sugar level in larvae of this species, apart from myotropic activity. Zopat-SK-1 showed myostimulatory action on the isolated hindgut of the adult beetles, but it decreased contractions of the heart, ejaculatory duct and oviduct. Injections of this peptide at a dose of 2 μg also caused delayed cardioinhibitory effects on the heartbeat of the pupae. Together with the ability to increase free sugar level in the haemolymph of larvae these were new physiological activities of sulfakinins in insects. Zopat-NVPL-4trunc. inhibited the muscle contractions of the two organs: hindgut and ejaculatory duct but it was inactive on the oviduct and the heart of the adult beetles. This peptide also increased free sugar level concentration in the haemolymph of Z. atratus larvae. These physiological actions are the first biological activities discovered for this group of the insect peptides. The present work showed pleiotropic activity of three neuropeptides and indicates that the visceral muscle contractions and the haemolymph sugar homeostasis in Z. atratus are regulated by complex mechanisms.

KeywordsInsect neuropeptides Zophobas atratus beetle Myotropic activity Communicated by G. Heldmaier.

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Author: Pawel Marciniak - Mariola Kuczer - Grzegorz Rosinski


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