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Cellular and Molecular Life Sciences

, Volume 68, Issue 16, pp 2797–2809

First Online: 24 November 2010Received: 01 June 2010Revised: 31 October 2010Accepted: 05 November 2010DOI: 10.1007-s00018-010-0593-2

Cite this article as: Chou, MY., Hung, JC., Wu, LC. et al. Cell. Mol. Life Sci. 2011 68: 2797. doi:10.1007-s00018-010-0593-2


The present study using zebrafish as a model explores the role of isotocin, a homolog of oxytocin, in controlling ion regulatory mechanisms. Double-deionized water treatment for 24 h significantly stimulated isotocin mRNA expression in zebrafish embryos. Whole-body Cl, Ca, and Na contents, mRNA expressions of ion transporters and ionocyte-differentiation related transcription factors, and the number of skin ionocytes decreased in isotocin morphants. In contrast, overexpression of isotocin caused an increase in ionocyte numbers. Isotocin morpholino caused significant suppression of foxi3a mRNA expression, while isotocin cRNA stimulated foxi3a mRNA expressions at the tail-bud stage of zebrafish embryos. The density of P63 an epidermal stem cell marker-positive cells was downregulated by isotocin morpholinos and was upregulated by isotocin cRNA. Taken together, isotocin stimulates the proliferation of epidermal stem cells and differentiation of ionocyte progenitors by regulating the P63 and Foxi3a transcription factors, consequently enhancing the functional activities of ionocytes.

KeywordsIsotocin Ionocyte Zebrafish Ion Differentiation M.-Y. Chou and J.-C. Hung contributed equally to this work.

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Autor: Ming-Yi Chou - Jo-Chi Hung - Liang-Chun Wu - Sheng-Ping L. Hwang - Pung-Pung Hwang


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