Association of CD99 short and long forms with MHC class I, MHC class II and tetraspanin CD81 and recruitment into immunological synapsesReportar como inadecuado




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BMC Research Notes

, 4:293

First Online: 13 August 2011Received: 01 June 2011Accepted: 13 August 2011DOI: 10.1186-1756-0500-4-293

Cite this article as: Pata, S., Otáhal, P., Brdička, T. et al. BMC Res Notes 2011 4: 293. doi:10.1186-1756-0500-4-293

Abstract

BackgroundCD99, a leukocyte surface glycoprotein, is broadly expressed in many cell types. On the cell surface, CD99 is expressed as two distinct isoforms, a long form and a short form. CD99 has been demonstrated to play a key role in several biological processes, including the regulation of T cell activation. However, the molecular mechanisms by which CD99 participates in such processes are unclear. As CD99 contains a short cytoplasmic tail, it is unlikely that CD99 itself takes part in its multi-functions. Association of CD99 with other membrane proteins has been suggested to be necessary for exerting its functions.

ResultsIn this study, we analyzed the association of CD99 with other cell surface molecules involved in T cell activation. We demonstrate the association of MHC class I, MHC class II and tetraspanin CD81 with CD99 molecules on the cell surface. Association of CD99 with its partners was observed for both isoforms. In addition, we determined that CD99 is a lipid raft-associated membrane protein and is recruited into the immunologic synapse during T cell activation. The implication of CD99 on T cell activation was investigated. Inhibition of anti-CD3 induced T cell proliferation by an anti-CD99 monoclonal antibody was observed.

ConclusionsWe provide evidence that CD99 directly interact and form the complex with the MHC class I and II, and tetraspanin CD81, and is functionally linked to the formation of the immunologic synapse. Upon T cell activation, CD99 engagement can inhibit T cell proliferation. We speculate that the CD99-MHC-CD81 complex is a tetraspanin web that plays an important role in T cell activation.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-4-293 contains supplementary material, which is available to authorized users.

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Autor: Supansa Pata - Pavel Otáhal - Tomáš Brdička - Witida Laopajon - Kodchakorn Mahasongkram - Watchara Kasinrerk

Fuente: https://link.springer.com/







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