Prolonged morphine administration alters protein expression in the rat myocardiumReportar como inadecuado

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Journal of Biomedical Science

, 18:89

First Online: 30 November 2011Received: 09 September 2011Accepted: 30 November 2011DOI: 10.1186-1423-0127-18-89

Cite this article as: Drastichova, Z., Skrabalova, J., Neckar, J. et al. J Biomed Sci 2011 18: 89. doi:10.1186-1423-0127-18-89


BackgroundMorphine is used in clinical practice as a highly effective painkiller as well as the drug of choice for treatment of certain heart diseases. However, there is lack of information about its effect on protein expression in the heart. Therefore, here we aimed to identify the presumed alterations in rat myocardial protein levels after prolonged morphine treatment.

MethodsMorphine was administered to adult male Wistar rats in high doses 10 mg-kg per day for 10 days. Proteins from the plasma membrane- and mitochondria-enriched fractions or cytosolic proteins isolated from left ventricles were run on 2D gel electrophoresis, scanned and quantified with specific software to reveal differentially expressed proteins.

ResultsNine proteins were found to show markedly altered expression levels in samples from morphine-treaded rats and these proteins were identified by mass spectrometric analysis. They belong to different cell pathways including signaling, cytoprotective, and structural elements.

ConclusionsThe present identification of several important myocardial proteins altered by prolonged morphine treatment points to global effects of this drug on heart tissue. These findings represent an initial step toward a more complex view on the action of morphine on the heart.

Keywordsrat myocardium morphine proteomics Electronic supplementary materialThe online version of this article doi:10.1186-1423-0127-18-89 contains supplementary material, which is available to authorized users.

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Autor: Zdenka Drastichova - Jitka Skrabalova - Jan Neckar - Frantisek Kolar - Jiri Novotny


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