Healing of Long-term Frozen Orthotopic Bone Allografts is not Affected by MHC Differences Between Donor and RecipientReportar como inadecuado

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Clinical Orthopaedics and Related Research®

, Volume 469, Issue 5, pp 1479–1486

First Online: 03 February 2011Received: 06 April 2010Accepted: 13 December 2010DOI: 10.1007-s11999-011-1796-z

Cite this article as: Reikerås, O., Reinholt, F.P., Zinöcker, S. et al. Clin Orthop Relat Res 2011 469: 1479. doi:10.1007-s11999-011-1796-z


BackgroundThe use of bone grafting in orthopaedic surgery has increased dramatically in recent years. However, the degree to which immune responses are important for the survival of the allograft is not fully understood. In particular it remains unclear whether differences in the major histocompatibility complex MHC influence incorporation of bone allografts and their subsequent biologic performance.

Questions-purposesTherefore, we asked whether isolated mismatch for MHC antigens of deep frozen bone allografts in the long-term causes 1 immune reactions, and whether these reactions have any effect on 2 morphologic features of the graft, 3 radiographic graft healing, and 4 graft strength.

MethodsWe used an established orthotopic tibial segment transplantation technique that allows determination of mechanical strength, histologic evaluation, and immune responses. Tibial segments that had been deep-frozen at −80°C for 1 year were transplanted into 24 PVG RT1 rats from either 12 syngeneic donors or 12 MHC congenic donors PVG.1U RT1. We determined immune responses using an indirect Coombs reaction and determined graft healing radiographically and mechanically after 6 months.

ResultsWe detected no alloantibody production to graft MHC-I antigens, and found no differences between syngeneic and MHC mismatched grafts in terms of remodeling with host bone, graft healing, and mechanical strength.

ConclusionsMismatches for MHC antigens do not seem to play a decisive role in healing of long-term, deep-frozen bone allografts.

Each author certifies that he or she has no commercial associations eg, consultancies, stock ownership, equity interest, patent-licensing arrangements, etc that might pose a conflict of interest in connection with the submitted article.

Each author certifies that his or her institution approved the animal protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research.

This work was performed at the Oslo University Hospital.

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Autor: Olav Reikerås - Finn P. Reinholt - Severin Zinöcker - Hamid Shegarfi - Bent Rolstad

Fuente: https://link.springer.com/

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