Expression of Nucleophosmin-NPM1 correlates with migration and invasiveness of colon cancer cellsReportar como inadecuado

Expression of Nucleophosmin-NPM1 correlates with migration and invasiveness of colon cancer cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Biomedical Science

, 19:53

First Online: 25 May 2012Received: 08 January 2012Accepted: 25 May 2012DOI: 10.1186-1423-0127-19-53

Cite this article as: Liu, Y., Zhang, F., Zhang, X. et al. J Biomed Sci 2012 19: 53. doi:10.1186-1423-0127-19-53


BackgroundWe aimed to examine the expression level of Nucleophosmin NPM1 protein in colon cancer tissues and to investigate the potential role of NPM1 in the regulation of cell migration and invasiveness.

MethodsImmunohistochemical assay was performed to examine the expression pattern of NPM1 in 31 groups of colonic carcinoma samples, including colon tumors, adjacent normal tissues, and matched metastatic lymph nodes from the same patients. Small interfering RNA technique and exogenous expression of wild type NPM1 methods were used to further verify the function of NPM1.

ResultsHigh-expression of NPM1 correlates with lymph node metastasis P = 0.0003 and poor survival rate of human colon cancer patients P = 0.017. SiRNA-mediated reduction of NPM1 was also shown to inhibit the migration and invasiveness of metastatic colon cancer HCT116 cell line. In addition, the exogenous expression of NPM1 in HT29 cells, a NPM1 low expression and low invasive colon cancer cell line, enhanced cell migration and invasiveness along with increased cell proliferation.

ConclusionsThe current study uncovered the critical role of NPM1 in the regulation of colon cancer cells migration and invasion, and NPM1 may serve as a potential marker for the prognosis of colon cancer patients.

KeywordsHuman colon cancer Nucleophosmin NPM1 Invasion Migration AbbreviationsNPM1Nucleophosmin:

AMLacute myeloid leukemia

PCNAproliferating cell nuclear antigen.

Electronic supplementary materialThe online version of this article doi:10.1186-1423-0127-19-53 contains supplementary material, which is available to authorized users.

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Autor: Yan Liu - Fei Zhang - Xiao-fang Zhang - Li-sha Qi - Lei Yang - Hua Guo - Ning Zhang


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