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BMC Research Notes

, 4:88

First Online: 29 March 2011Received: 15 December 2010Accepted: 29 March 2011DOI: 10.1186-1756-0500-4-88

Cite this article as: Jantzen, S.G., Sanderson, D.S., von Schalburg, K.R. et al. BMC Res Notes 2011 4: 88. doi:10.1186-1756-0500-4-88

Abstract

BackgroundAtlantic salmon Salmo salar L. is an environmentally and economically important organism and its gene content is reasonably well characterized. From a transcriptional standpoint, it is important to characterize the changes in gene expression over the course of unperturbed early development, from fertilization through to the parr stage.

FindingsS. salar samples were taken at 17 time points from 2 to 89 days post fertilization. Total RNA was extracted and cRNA was synthesized and hybridized to a newly developed 44K oligo salmonid microarray platform. Quantified results were subjected to preliminary data analysis and submitted to NCBI-s Gene Expression Omnibus GEO. Data can be found under the GEO accession number GSE25938. http:-www.ncbi.nlm.nih.gov-geo-query-acc.cgi?acc=GSE25938

ConclusionsThroughout the entire period of development, several thousand genes were found to be differentially regulated. This work represents the trancriptional characterization of a very large geneset that will be extremely valuable in further examination of the transcriptional changes in Atlantic salmon during the first few months of development. The expression profiles can help to annotate salmon genes in addition to being used as references against any number of experimental variables to which developing salmonids might be subjected.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-4-88 contains supplementary material, which is available to authorized users.

Stuart G Jantzen, Dan S Sanderson contributed equally to this work.

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Autor: Stuart G Jantzen - Dan S Sanderson - Kris R von Schalburg - Motoshige Yasuike - Francesco Marass - Ben F Koop

Fuente: https://link.springer.com/







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