Levels and values of circulating endothelial progenitor cells, soluble angiogenic factors, and mononuclear cell apoptosis in liver cirrhosis patientsReportar como inadecuado




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Journal of Biomedical Science

, 19:66

First Online: 18 July 2012Received: 17 January 2012Accepted: 18 July 2012DOI: 10.1186-1423-0127-19-66

Cite this article as: Chen, CH., Chang, LT., Tung, WC. et al. J Biomed Sci 2012 19: 66. doi:10.1186-1423-0127-19-66

Abstract

BackgroundThe roles of circulating endothelial progenitor cell EPC and mononuclear cell apoptosis MCA in liver cirrhosis LC patients are unknown. Moreover, vascular endothelial growth factor VEGF and stromal cell-derived factor SDF-1α are powerful endogenous substances enhancing EPC migration into circulation. We assessed the level and function of EPCs CD31-CD34 E1, KDR-CD34 E2, CXCR4-CD34 E3, levels of MCA, VEGF and SDF-1α in circulation of LC patients.

MethodsBlood sample was prospectively collected once for assessing EPC level and function, MCA, and plasma levels of VEGF and SDF-1α using flow cytometry and enzyme-linked immunosorbent assay ELISA, respectively, in 78 LC patients and 25 age- and gender-matched healthy controls.

ResultsNumber of EPCs E1, E2, E3 was lower all p < 0.0001, whereas SDF-1α level and MCA were higher p < 0.001 in study patients compared with healthy controls. Number of EPCs E2, E3 was higher but MCA was lower all p < 0.05 in Child-s class A compared with Child-s class B and C patients, although no difference in VEGF and SDF-1α levels were noted among these patients. Chronic hepatitis B and esophageal varices bleeding were independently, whereas chronic hepatitis C, elevated aspartate aminotransferase AST, and decompensated LC were inversely and independently correlated with circulating EPC level all p < 0.03. Additionally, angiogenesis and transwell migratory ability of EPCs were reduced in LC patients than in controls all p < 0.001.

ConclusionThe results of this study demonstrated that level, angiogenic capacity, and function of circulating EPCs were significantly reduced, whereas plasma levels of SDF-1α and circulating MCA were substantially enhanced in cirrhotic patients.

KeywordsLiver cirrhosis Circulating endothelial progenitor cells Angiogenesis factors Cellular apoptosis Electronic supplementary materialThe online version of this article doi:10.1186-1423-0127-19-66 contains supplementary material, which is available to authorized users.

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Autor: Chih-Hung Chen - Li-Teh Chang - Wei-Chih Tung - Yung-Lung Chen - Chia-Lo Chang - Steve Leu - Cheuk-Kwan Sun - Tzu-Hsien Ts

Fuente: https://link.springer.com/



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