Three new chondrosarcoma cell lines: one grade III conventional central chondrosarcoma and two dedifferentiated chondrosarcomas of boneReportar como inadecuado




Three new chondrosarcoma cell lines: one grade III conventional central chondrosarcoma and two dedifferentiated chondrosarcomas of bone - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Cancer

, 12:375

Cell and molecular biology

Abstract

BackgroundChondrosarcoma is the second most common primary sarcoma of bone. High-grade conventional chondrosarcoma and dedifferentiated chondrosarcoma have a poor outcome. In pre-clinical research aiming at the identification of novel treatment targets, the need for representative cell lines and model systems is high, but availability is scarce.

MethodsWe developed and characterized three cell lines, derived from conventional grade III chondrosarcoma L835, and dedifferentiated chondrosarcoma L2975 and L3252 of bone. Proliferation and migration were studied and we used COBRA-FISH and array-CGH for karyotyping and genotyping. Immunohistochemistry for p16 and p53 was performed as well as TP53 and IDH mutation analysis. Cells were injected into nude mice to establish their tumorigenic potential.

ResultsWe show that the three cell lines have distinct migrative properties, L2975 had the highest migration rate and showed tumorigenic potential in mice. All cell lines showed chromosomal rearrangements with complex karyotypes and genotypic aberrations were conserved throughout late passaging of the cell lines. All cell lines showed loss of CDKN2A, while TP53 was wild type for exons 5–8. L835 has an IDH1 R132C mutation, L2975 an IDH2 R172W mutation and L3252 is IDH wild type.

ConclusionsBased on the stable culturing properties of these cell lines and their genotypic profile resembling the original tumors, these cell lines should provide useful functional models to further characterize chondrosarcoma and to evaluate new treatment strategies.

KeywordsBone neoplasm Chondrosarcoma Cell line IDH1 IDH2 p16 Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-12-375 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Jolieke G van Oosterwijk - Danielle de Jong - Maayke AJH van Ruler - Pancras CW Hogendoorn - PD Sander Dijkstra - Carla 

Fuente: https://link.springer.com/



DESCARGAR PDF




Documentos relacionados