Near-infrared low-level laser stimulation of telocytes from human myometriumReportar como inadecuado

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Lasers in Medical Science

, Volume 29, Issue 6, pp 1867–1874

First Online: 29 May 2014Received: 15 January 2014Accepted: 24 April 2014DOI: 10.1007-s10103-014-1589-1

Cite this article as: Campeanu, RA., Radu, B.M., Cretoiu, S.M. et al. Lasers Med Sci 2014 29: 1867. doi:10.1007-s10103-014-1589-1


Telocytes TCs are a brand-new cell type frequently observed in the interstitial space of many organs see TCs are defined by very long tens of micrometers and slender prolongations named telopodes. At their level, dilations—called podoms ~300 nm, alternate with podomers 80–100 nm. TCs were identified in a myometrial interstitial cell culture based on morphological criteria and by CD34 and PDGF receptor alpha PDGFRα immunopositivity. However, the mechanisms of telopodes formation and-or elongation and ramification is not known. We report here the low-level laser stimulation LLLS using a 1,064-nm neodymium-doped yttrium aluminum garnet Nd:YAG laser with an output power of 60 mW of the telopodal lateral extension TLE growth in cell culture. LLLS of TCs determines a higher growth rate of TLE in pregnant myometrium primary cultures 10.3 ± 1.0 μm-min compared to nonpregnant ones 6.6 ± 0.9 μm-min. Acute exposure 30 min of TCs from pregnant myometrium to 1 μM mibefradil, a selective inhibitor of T-type calcium channels, determines a significant reduction in the LLLS TLE growth rate 5.7 ± 0.8 μm-min compared to LLLS per se in same type of samples. Meanwhile, chronic exposure 24 h completely abolishes the LLLS TLE growth in both nonpregnant and pregnant myometria. The initial direction of TLE growth was modified by LLLS, the angle of deviation being more accentuated in TCs from human pregnant myometrium than in TCs from nonpregnant myometrium. In conclusion, TCs from pregnant myometrium are more susceptible of reacting to LLLS than those from nonpregnant myometrium. Therefore, some implications are emerging for low-level laser therapy LLLT in uterine regenerative medicine.

Keywords1064 Nd:YAG laser Low-level laser stimulation Telocytes Telopodes Human myometrium Pregnancy Razvan-Alexandru Campeanu and Beatrice Mihaela Radu contributed equally to this work.

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Autor: Razvan-Alexandru Campeanu - Beatrice Mihaela Radu - Sanda Maria Cretoiu - Daniel Dumitru Banciu - Adela Banciu - Dragos C


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