Genetic polymorphisms of glutathione S-transferase M1 and T1, and evaluation of oxidative stress in patients with non-small cell lung cancerReportar como inadecuado




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European Journal of Medical Research

, 19:67

First Online: 04 December 2014Received: 29 January 2014Accepted: 17 November 2014DOI: 10.1186-s40001-014-0067-3

Cite this article as: Zhang, H., Wu, X., Xiao, Y. et al. Eur J Med Res 2014 19: 67. doi:10.1186-s40001-014-0067-3

Abstract

BackgroundOur objective is to investigate the genetic polymorphisms of the glutathione S-transferase M1 and T1 genes GSTM1 and GSTT1 and evaluate oxidative damage in patients with non-small lung cancer N-SCLC.

MethodsOne hundred and ten patients with N-SCLC and 100 controls are included in this case-control study. Multiplex polymerase chain reaction PCR analyses were used to identify the genotypes. The activities of malondialdehyde MDA and nitric oxide NO and total antioxidant capacity T-AOC were detected by spectroscopic analysis.

ResultsThe frequencies of the GSTM1, T1, and GSTM1-T1 null genotypes in the patient group were significantly higher than that in the control group OR = 2.071, P = 0.009; OR = 1.900, P = 0.024; OR = 3.258, P = 0.003. The activities of MDA and NO were significantly higher in the patient group than that in the control group P <0.001, and T-AOC was significantly lower in patient group than that in control group P <0.001. The activities of MDA, and NO were higher but the T-AOC was lower in patients with the GSTM1, T1 and M1-T1 null genotypes than those in patients with GSTM1, T1 and M1-T1 present genotypes P <0.001.

ConclusionsOur results suggest that oxidative damage may be play a important role in patients with N-SCLC, and that GSTM1 and GSTT1 null genotypes may predispose the cells of patients with N-SCLC to increased oxidative damage.

KeywordsGlutathione S-transferases Polymorphism Oxidative stress Non-small lung cancer AbbreviationsGSTM1glutathione S-transferase M1

GSTT1glutathione S-transferase T1

N-SCLCnon-small lung cancer

PCRmultiplex polymerase chain reaction

OSoxidative stress

MDAmalondialdehyde

NOnitric oxide

T-AOCtotal antioxidant capacity

Electronic supplementary materialThe online version of this article doi:10.1186-s40001-014-0067-3 contains supplementary material, which is available to authorized users.

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Autor: Hongyan Zhang - Xuwei Wu - Yi Xiao - Mei Chen - Zhidong Li - Xing Wei - Kaifa Tang

Fuente: https://link.springer.com/







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