Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of strokeReportar como inadecuado




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Journal of Translational Medicine

, 8:77

First Online: 20 August 2010Received: 08 November 2009Accepted: 20 August 2010DOI: 10.1186-1479-5876-8-77

Cite this article as: Sun, C., Zhang, H., Li, J. et al. J Transl Med 2010 8: 77. doi:10.1186-1479-5876-8-77

Abstract

BackgroundThe relationship between functional improvements in ischemic rats given a neural stem cell NSC transplant and the modulation of the class I major histocompatibility complex MHC mediated by NSC-derived neurotrophins was investigated.

MethodsThe levels of gene expression of nerve growth factor NGF, brain-derived neurotropic factor BDNF and neurotrophin-3 NT-3 were assayed from cultures of cortical NSC from Sprague-Dawley rat E16 embryos. The levels of translated NGF in spent culture media from NSC cultures and the cerebral spinal fluid CSF of rats with and without NGF injection or NSC transplant were also measured.

ResultsWe found a significant increase of NGF, BDNF and NT-3 transcripts and NGF proteins in both the NSC cultures and the CSF of the rats. The immunochemical staining for MHC in brain sections and the enzyme-linked immunosorbent assay of CSF were carried out in sham-operated rats and rats with surgically induced focal cerebral ischemia. These groups were further divided into animals that did and did not receive NGF administration or NSC transplant into the cisterna magna. Our results show an up-regulation of class I MHC in the ischemic rats with NGF and NSC administration. The extent of caspase-III immunoreactivity was comparable among three arms in the ischemic rats.

ConclusionReadouts of somatosensory evoked potential and the trap channel test illustrated improvements in the neurological function of ischemic rats treated with NGF administration and NSC transplant.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-8-77 contains supplementary material, which is available to authorized users.

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Autor: Chongran Sun - Han Zhang - Jin Li - Hua Huang - Hongbin Cheng - Yajie Wang - Ping Li - Yihua An

Fuente: https://link.springer.com/



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