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Metabolomics

, Volume 9, Supplement 1, pp 121–131

First Online: 31 May 2011Received: 16 February 2011Accepted: 12 May 2011DOI: 10.1007-s11306-011-0318-z

Cite this article as: Okazaki, Y., Kamide, Y., Hirai, M.Y. et al. Metabolomics 2013 9Suppl 1: 121. doi:10.1007-s11306-011-0318-z

Abstract

Plants synthesize a wide range of hydrophobic compounds, generally known as lipids. Here, we report an application of liquid chromatography ion trap time-of-flight mass spectrometry LC-IT-TOF-MS for plant lipidomics. Using hydrophilic interaction chromatography HILIC for class separation, typical membrane lipids including glycerolipids, steryl glucosides and glucosylceramides, and hydrophobic plant secondary metabolites such as saponins were analyzed simultaneously. By this method, we annotated approximately 100 molecules from Arabidopsis thaliana. To demonstrate the application of this method to biological study, we analyzed Arabidopsis mutant trigalactosyldiacylglycerol3 tgd3, which has a complex metabolic phenotype including the accumulation of unusual forms of galactolipids. Lipid profiling by LC-MS revealed that tgd3 accumulated an unusual form of digalactosyldiacylglycerol, annotated as Galβ1 → 6βGalDG. The compositional difference between normal and unusual forms of digalactosyldiacylglycerol was detected by this method. In addition, we analyzed well-known Arabidopsis mutants ats1-1, fad6-1, and fad7-2, which are also disrupted in lipid metabolic genes. Untargeted lipidome analysis coupled with multivariate analysis clearly discriminated the mutants and their distinctive metabolites. These results indicated that HILIC-MS is an efficient method for plant lipidomics.

KeywordsHydrophilic interaction chromatography Ion trap time-of-flight mass spectrometry Lipidome Lipidomics Electronic supplementary materialThe online version of this article doi:10.1007-s11306-011-0318-z contains supplementary material, which is available to authorized users.

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Autor: Yozo Okazaki - Yukiko Kamide - Masami Yokota Hirai - Kazuki Saito

Fuente: https://link.springer.com/







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