Dermal exposure to immunostimulants induces changes in activity and proliferation of coelomocytes of Eisenia andreiReportar como inadecuado




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Journal of Comparative Physiology B

, Volume 183, Issue 3, pp 313–322

First Online: 27 September 2012Received: 07 February 2012Revised: 23 August 2012Accepted: 28 August 2012DOI: 10.1007-s00360-012-0710-7

Cite this article as: Homa, J., Zorska, A., Wesolowski, D. et al. J Comp Physiol B 2013 183: 313. doi:10.1007-s00360-012-0710-7

Abstract

Due to the specific habitat conditions in which they live, earthworms are constantly exposed to pathogens. Consequently, they have evolved various immuno-defense mechanisms, including cellular coelomocytes and humoral responses, which may help to eliminate deleterious micro-organisms but also repair and-or protect host cells and tissues. Similar to mammalian phagocytes, coelomocytes can kill ingested pathogens with reactive oxygen species ROS and nitric oxide. In the present work, we studied the effects of the dermal exposure of Eisenia andrei earthworms to different immuno-stimulants: phorbol-12-myristate-13-acetate PMA, lipopolysaccharide LPS or concanavalin A ConA. After 3 days of treatment with all immuno-stimulants, decreased numbers and changed composition of the coelomocytes were observed. The immuno-stimulants also induced numerous changes in bactericidal activity, including ROS production. Furthermore, all stimulants increased cell proliferation while only LPS-treatment significantly elevated apoptosis of coelomocytes. These results demonstrate that in vivo treatment of earthworms with immuno-stimulants induces various changes in their coelomocyte response.

KeywordsCoelomocytes Immunostimulants Proliferation Heat shock protein 72 Nitric oxide Respiratory burst Communicated by G. Heldmaier.

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Autor: Joanna Homa - Anna Zorska - Dawid Wesolowski - Magdalena Chadzinska

Fuente: https://link.springer.com/







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