Metabolomic profiling of lung and prostate tumor tissues by capillary electrophoresis time-of-flight mass spectrometryReport as inadecuate

Metabolomic profiling of lung and prostate tumor tissues by capillary electrophoresis time-of-flight mass spectrometry - Download this document for free, or read online. Document in PDF available to download.


, Volume 9, Issue 2, pp 444–453

First Online: 02 November 2012Received: 11 June 2012Accepted: 27 July 2012DOI: 10.1007-s11306-012-0452-2

Cite this article as: Kami, K., Fujimori, T., Sato, H. et al. Metabolomics 2013 9: 444. doi:10.1007-s11306-012-0452-2


Metabolic microenvironment of tumor cells is influenced by oncogenic signaling and tissue-specific metabolic demands, blood supply, and enzyme expression. To elucidate tumor-specific metabolism, we compared the metabolomics of normal and tumor tissues surgically resected pairwise from nine lung and seven prostate cancer patients, using capillary electrophoresis time-of-flight mass spectrometry CE-TOFMS. Phosphorylation levels of enzymes involved in central carbon metabolism were also quantified. Metabolomic profiles of lung and prostate tissues comprised 114 and 86 metabolites, respectively, and the profiles not only well distinguished tumor from normal tissues, but also squamous cell carcinoma from the other tumor types in lung cancer and poorly differentiated tumors from moderately differentiated tumors in prostate cancer. Concentrations of most amino acids, especially branched-chain amino acids, were significantly higher in tumor tissues, independent of organ type, but of essential amino acids were particularly higher in poorly differentiated than moderately differentiated prostate cancers. Organ-dependent differences were prominent at the levels of glycolytic and tricarboxylic acid cycle intermediates and associated energy status. Significantly high lactate concentrations and elevated activating phosphorylation levels of phosphofructokinase and pyruvate kinase in lung tumors confirmed hyperactive glycolysis. We highlighted the potential of CE-TOFMS-based metabolomics combined with phosphorylated enzyme analysis for understanding tissue-specific tumor microenvironments, which may lead to the development of more effective and specific anticancer therapeutics.

KeywordsMetabolomics CE-MS Phosphoproteomics Lung cancer Prostate cancer Tumor microenvironment Kenjiro Kami and Tamaki Fujimori contributed equally to this study.

Electronic supplementary materialThe online version of this article doi:10.1007-s11306-012-0452-2 contains supplementary material, which is available to authorized users.

Download fulltext PDF

Author: Kenjiro Kami - Tamaki Fujimori - Hajime Sato - Mutsuko Sato - Hiroyuki Yamamoto - Yoshiaki Ohashi - Naoyuki Sugiyama - Yasu


Related documents