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BMC Cancer

, 10:311

First Online: 21 June 2010Received: 17 December 2009Accepted: 21 June 2010DOI: 10.1186-1471-2407-10-311

Cite this article as: Nakayama, T., Ling, ZQ., Mukaisho, K. et al. BMC Cancer 2010 10: 311. doi:10.1186-1471-2407-10-311

Abstract

BackgroundEradication of early gastric carcinoma GC is thought to contribute to reduction in the mortality of GC, given that most of the early GCs progress to the advanced GCs. However, early GC is alternatively considered a dormant variant of GC, and it infrequently progresses to advanced GC. The aim of this study was to clarify the extent of overlap of genetic lineages between early and advanced tubular adenocarcinomas TUBs of the stomach.

MethodsImmunohistochemical staining for p53 was performed using 28 surgically resected stomachs with 13 intramucosal and 15 invasive TUBs. By chromosome- and array-based comparative genomic hybridization CGH, genomic copy number constitution was compared between the mucosal and invasive parts of the invasive TUBs and between the mucosal parts of the invasive and intramucosal TUBs, using 25 and 22 TUBs, respectively. TP53 mutation in exons 5-8 was examined in 20 TUBs.

ResultsChromosomal CGH revealed that 4q+ and 11q+ were more common in advanced and early TUBs, respectively, whereas copy number changes in 8q and 17p showed no significant differences between early and advanced TUBs. However, array CGH revealed that, of the 13 intramucosal TUBs examined, loss of MYC MYC- and gain of TP53 TP53+ was detected in 9 TUBs and MYC+ and-or TP53- was detected in 3 TUBs. Of the mucosal samples of 9 invasive TUBs, 7 showed MYC-TP53+ and none showed MYC+ and-or TP53-. Of the 9 samples from the invasive parts, 1 from submucosal cancers showed MYC-TP53+ and 6 1 from submucosal and 5 from advanced cancers showed MYC+ and-or TP53-. The latter 6 tumours commonly showed a mutant pattern diffuse or null in p53 immunohistochemistry, and 4 of the 6 tumours assessable for TP53 sequence analysis revealed mutations. The overall array CGH pattern indicated that, between the mucosal and invasive parts, genetic lineage was found discontinuous in 5 advanced cancers and continuous in 3 submucosal cancers.

ConclusionsGenetic lineages often differed between early and advanced TUBs. MYC-TP53+ and MYC + and-or TP53- may be the signatures of dormant and aggressive TUBs, respectively, in the stomach.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-10-311 contains supplementary material, which is available to authorized users.

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Autor: Takahisa Nakayama - Zhi-Qiang Ling - Ken-ichi Mukaisho - Takanori Hattori - Hiroyuki Sugihara

Fuente: https://link.springer.com/







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