Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbitsReportar como inadecuado




Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Translational Medicine

, 8:40

Immunovirology

Abstract

BackgroundAround half million new cases of cervical cancer arise each year, making the development of an effective therapeutic vaccine against HPV a high priority. As the E6 and E7 oncoproteins are expressed in all HPV-16 tumour cells, vaccines expressing these proteins might clear an already established tumour and support the treatment of HPV-related precancerous lesions.

MethodsThree different immunisation regimens were tested in a pre-clinical trial in rabbits to evaluate the humoral and cell-mediated responses of a putative HPV-16 vaccine. Fowlpoxvirus FP recombinants separately expressing the HPV-16 E6 FPE6 and E7 FPE7 transgenes were used for priming, followed by E7 protein boosting.

ResultsAll of the protocols were effective in eliciting a high antibody response. This was also confirmed by interleukin-4 production, which increased after simultaneous priming with both FPE6 and FPE7 and after E7 protein boost. A cell-mediated immune response was also detected in most of the animals.

ConclusionThese results establish a preliminary profile for the therapy with the combined use of avipox recombinants, which may represent safer immunogens than vaccinia-based vectors in immuno-compromised individuals, as they express the transgenes in most mammalian cells in the absence of a productive replication.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-8-40 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Antonia Radaelli - Eleana Pozzi - Sole Pacchioni - Carlo Zanotto - Carlo De Giuli Morghen

Fuente: https://link.springer.com/







Documentos relacionados