Increased unsaturation of lipids in cytoplasmic lipid droplets in DAOY cancer cells in response to cisplatin treatmentReportar como inadecuado




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Metabolomics

, Volume 9, Issue 3, pp 722–729

First Online: 14 December 2012Received: 13 August 2012Accepted: 19 November 2012DOI: 10.1007-s11306-012-0483-8

Cite this article as: Pan, X., Wilson, M., McConville, C. et al. Metabolomics 2013 9: 722. doi:10.1007-s11306-012-0483-8

Abstract

Increases in H nuclear magnetic resonance spectroscopy NMR visible lipids are a well-documented sign of treatment response in cancers. Lipids in cytoplasmic lipid droplets LDs are the main contributors to the NMR lipid signals. Two human primitive neuroectodermal tumour cell lines with different sensitivities to cisplatin treatment were studied. Increases in NMR visible saturated and unsaturated lipids in cisplatin treated DAOY cells were associated with the accumulation of LDs prior to DNA fragmentation due to apoptosis. An increase in unsaturated fatty acids UFAs was detected in isolated LDs from DAOY cells, in contrast to a slight decrease in UFAs in lipid extracts from whole cells. Oleic acid and linoleic acid were identified as the accumulating UFAs in LDs by heteronuclear single quantum coherence spectroscopy HSQC. H NMR lipids in non-responding PFSK-1 cells were unchanged by exposure to 10 μM cisplatin. These findings support the potential of NMR detectable UFAs to serve as a non-invasive marker of tumour cell response to treatment.

KeywordsLipid droplets H NMR Isolation Cisplatin  Download fulltext PDF



Autor: Xiaoyan Pan - Martin Wilson - Carmel McConville - Theodoros N. Arvanitis - Julian L. Griffin - Risto A. Kauppinen - Andre

Fuente: https://link.springer.com/







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