Conjugation, characterization and toxicity of lipophosphoglycan-polyacrylic acid conjugate for vaccination against leishmaniasisReportar como inadecuado




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Journal of Biomedical Science

, 20:35

First Online: 03 June 2013Received: 06 February 2013Accepted: 16 May 2013DOI: 10.1186-1423-0127-20-35

Cite this article as: Topuzogullari, M., Cakir Koc, R., Dincer Isoglu, S. et al. J Biomed Sci 2013 20: 35. doi:10.1186-1423-0127-20-35

Abstract

Research on the conjugates of synthetic polyelectrolytes with antigenic molecules, such as proteins, peptides, or carbohydrates, is an attractive area due to their highly immunogenic character in comparison to classical adjuvants. For example, polyacrylic acid PAA is a weak polyelectrolyte and has been used in several biomedical applications such as immunological studies, drug delivery, and enzyme immobilization. However, to our knowledge, there are no studies that document immune-stimulant properties of PAA in Leishmania infection. Therefore, we aimed to develop a potential vaccine candidate against leishmaniasis by covalently conjugating PAA with an immunologically vital molecule of lipophosphoglycan LPG found in Leishmania parasites. In the study, LPG and PAA were conjugated by a multi-step procedure, and final products were analyzed with GPC and MALDI-TOF MS techniques. In cytotoxicity experiments, LPG-PAA conjugates did not indicate toxic effects on L929 and J774 murine macrophage cells. We assume that LPG-PAA conjugate can be a potential vaccine candidate, and will be immunologically characterized in further studies to prove its potential.

KeywordsPolymer Vaccine Delivery Adjuvant Leishmania Conjugation Electronic supplementary materialThe online version of this article doi:10.1186-1423-0127-20-35 contains supplementary material, which is available to authorized users.

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Autor: Murat Topuzogullari - Rabia Cakir Koc - Sevil Dincer Isoglu - Melahat Bagirova - Zeynep Akdeste - Serhat Elcicek - Olga N 

Fuente: https://link.springer.com/







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