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BMC Cancer

, 15:193

Translational oncologyRoles of microRNAs and other noncoding RNAs in cancer


BackgroundSince recent studies revealed the feasibility to detect blood-based microRNAs miRNAs, miRs in breast cancer BC patients a new field has been opened for circulating miRNAs as potential biomarkers in BC. In this pilot study, we evaluated to our knowledge for the first time whether distinct pattern of urinary miRNAs might be also applicable as innovative biomarkers for BC detection.

MethodsUrinary miRNA expression levels of nine BC-related miRNAs miR-21, miR-34a, miR-125b, miR-155, miR-195, miR-200b, miR-200c, miR-375, miR-451 from 24 untreated, primary BC patients and 24 healthy controls were quantified by realtime-PCR. The receiver operating characteristic analyses ROC and logistic regression were calculated to assess discriminatory accuracy.

ResultsSignificant differences were found in the expression of four BC-associated miRNAs quantified as median miRNA expression levels. Urinary miR-155 levels were significantly higher in BC patients compared to healthy controls 1.49vs.0.25; p < 0.001. In contrast, compared to healthy controls, BC patients exhibited significantly lower urinary expression levels of miR-21 2.27vs.5.07; p < 0.001, miR-125b 0.71vs.1.62; p < 0.001, and miR-451 0.02vs.0.59 p = 0.004, respectively. The ROC including all miRNAs as well as the group of the four significant deregulated miRNAs separated BC patients from healthy controls with a very high area under the receiver operating characteristic curve AUC = 0.932 and high accuracy AUC = 0.887, respectively.

ConclusionsWe were able to demonstrate for the first time the feasibility to detect distinct BC-dependent urinary miRNA profiles. The expression levels of four urinary miRNAs were specifically altered in our cohort of BC patients compared to healthy controls. This distinct pattern offers the possibility for a specific discrimination between healthy women and primary BC patients. This sustains the potential role of urinary miRNAs as non-invasive innovative urine-based biomarkers for BC detection.

KeywordsBreast cancer microRNA Urine Biomarker Non-invasive Innovative Discrimination AbbreviationsAUCArea under the curve

Bak1Bcl-2 antagonist killer1

BCBreast cancer

cDNAcomplementary DNA


HKGHousekeeping gene

MDR1-P-glycoproteinMultidrug resistance-1-P-glycoprotein

miRmiRNA, microRNA

MSUMidstream specimens of urine

PDCD4Programmed Cell Death 4

PTENPhosphatase and tensin homolog

qPCRquantitative polymerase chain reaction

qRT-PCRquantitative reverse transcriptase polymerase chain reaction

realtime-PCRrealtime polymerase chain reaction

RNARibonucleic acid

ROCReceiver Operating Characteristic

TPM1Tropomyosin alpha-1 chain.

Electronic supplementary materialThe online version of this article doi:10.1186-s12885-015-1190-4 contains supplementary material, which is available to authorized users.

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Autor: Thalia Erbes - Marc Hirschfeld - Gerta Rücker - Markus Jaeger - Jasmin Boas - Severine Iborra - Sebastian Mayer - Gerald 

Fuente: https://link.springer.com/

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