Differential sensitivity of polyhydroxyalkanoate producing bacteria to fermentation inhibitors and comparison of polyhydroxybutyrate production from Burkholderia cepacia and Pseudomonas pseudoflavaReportar como inadecuado




Differential sensitivity of polyhydroxyalkanoate producing bacteria to fermentation inhibitors and comparison of polyhydroxybutyrate production from Burkholderia cepacia and Pseudomonas pseudoflava - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Research Notes

, 6:219

First Online: 04 June 2013Received: 20 February 2013Accepted: 30 May 2013DOI: 10.1186-1756-0500-6-219

Cite this article as: Dietrich, D., Illman, B. & Crooks, C. BMC Res Notes 2013 6: 219. doi:10.1186-1756-0500-6-219

Abstract

BackgroundThe aim of this study is determine the relative sensitivity of a panel of seven polyhydroxyalkanoate producing bacteria to a panel of seven lignocellulosic-derived fermentation inhibitors representing aliphatic acids, furans and phenolics. A further aim was to measure the polyhydroxybutyrate production of select organisms on lignocellulosic-derived monosaccharides arabinose, xylose, glucose and mannose.

FindingsWe examined the sensitivity of seven polyhydroxyalkanoate producing bacteria: Azohydromonas lata, Bacillus megaterium, Bacillus cereus, Burkholderia cepacia, Pseudomonas olevorans, Pseudomonas pseudoflava and Ralstonia eutropha, against seven fermentation inhibitors produced by the saccharification of lignocellulose: acetic acid, levulinic acid, coumaric acid, ferulic acid, syringaldehyde, furfural, and hyroxymethyfurfural. There was significant variation in the sensitivity of these microbes to representative phenolics ranging from 0.25-1.5 g-L coumaric and ferulic acid and between 0.5-6.0 g-L syringaldehyde. Inhibition ranged from 0.37-4 g-L and 0.75-6 g-L with acetic acid and levulinic acid, respectively. B. cepacia and P. pseudoflava were selected for further analysis of polyhydroxyalkanoate production.

ConclusionsWe find significant differences in sensitivity to the fermentation inhibitors tested and find these variations to be over a relevant concentration range given the concentrations of inhibitors typically found in lignocellulosic hydrolysates. Of the seven bacteria tested, B. cepacia demonstrated the greatest inhibitor tolerance. Similarly, of two organisms examined for polyhydroxybutyrate production, B. cepacia was notably more efficient when fermenting pentose substrates.

KeywordsPolyhydroxyalkanoate Fermentation inhibitors Bioconversion Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-6-219 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Diane Dietrich - Barbara Illman - Casey Crooks

Fuente: https://link.springer.com/







Documentos relacionados