High expression of NQO1 is associated with poor prognosis in serous ovarian carcinomaReportar como inadecuado




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BMC Cancer

, 15:244

Translational oncology

Abstract

BackgroundNADPH:quinone oxidoreductase NQO1 is a flavoprotein that catalyzes two-electron reduction and detoxification of quinones and its derivatives. NQO1 catalyzes reactions that have a protective effect against redox cycling, oxidative stress and neoplasia. High expression of NQO1 is associated with many solid tumors including those affecting the colon, breast and pancreas; however, its role in the progression of ovarian carcinoma is largely undefined. This study aimed to investigate the clinicopathological significance of high NQO1 expression in serous ovarian carcinoma.

MethodsNQO1 protein expression was assessed using immunohistochemical IHC staining in 160 patients with serous ovarian carcinoma, 62 patients with ovarian borderline tumors and 53 patients with benign ovarian tumors. Quantitative real-time polymerase chain reaction qRT-PCR was performed to detect NQO1 mRNA expression levels. The correlation between high NQO1 expression and clinicopathological features of ovarian carcinoma was evaluated by Chi-square and Fisher’s exact test. Overall survival OS rates of all of ovarian carcinoma patients were calculated using the Kaplan-Meier method, and univariate and multivariate analyses were performed using the Cox proportional hazards regression model.

ResultsNQO1 protein expression in ovarian carcinoma cells was predominantly cytoplasmic. Strong, positive expression of NQO1 protein was observed in 63.8% 102-160 of ovarian carcinomas, which was significantly higher than in borderline serous tumors 32.3%, 20-62 or benign serous tumors 11.3%, 6-53. Importantly, the rate of strong, positive NQO1 expression in borderline serous tumors was also higher than in benign serous tumors. High expression of NQO1 protein was closely associated with higher histological grade, advanced clinical stage and lower OS rates in ovarian carcinomas. Moreover, multivariate analysis indicated that NQO1 was a significant independent prognostic factor, in addition to clinical stage, in patients with ovarian carcinoma.

ConclusionsNQO1 is frequently upregulated in ovarian carcinoma. High expressin of NQO1 protein may be an effective biomarker for poor prognostic evaluation of patients with serous ovarian carcinomas.

KeywordsOvarian carcinoma NQO1 Immunohistochemistry Survival analysis Xuelian Cui and Lianhua Li contributed equally to this work.

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Autor: Xuelian Cui - Lianhua Li - Guanghai Yan - Kai Meng - Zhenhua Lin - Yunze Nan - Guang Jin - Chunyu Li

Fuente: https://link.springer.com/







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